Fluorimètre QUBIT

Galerie et schémas techniques

Description

Caractéristiques

• Haute sensibilité – plus sensible que l’absorbance UV – quantification basée
• Précision et vitesse—quantifie avec précision l’ADN, l’ARN, ou protéines en moins de 3 secondes
• Idéal pour les échantillons précieux—nécessite aussi peu que 1 μL d’échantillon
• Réactifs et tubes optimisés—Invitrogen™ Les réactifs et les tubes à essai Qubit™ fonctionnent mieux avec Fluorimètres Qubit

Spécifications techniques

DMSO

Working

Poids

743 g; 2.1 lb

dsDNA

dsDNA concentrations and

Formats

standard assay and 1X assay

Product

concentration (ng/μL) range (ng) No. of assays Cat. No.

(10 μL)

(1–20 μL)

Attribut

Description

Platform

Qubit 4 Qubit 4, Qubit Flex

dsDNA BR

DNA assay kits.

75 assays

Q33221

Frequence

50/60 Hz

Oligo DNA

Oligo DNA

Standards

Samples

USB drive

USB drive

dye stock

solution

275 assays

Q33222

Long ssDNA

Long ssDNA

(W x L x H)

5.4 x 10 x 2.2 in. 7.3 x 11.1 x 4.1 in.

Data export

Wi-Fi Wi-Fi

solutions >

solutions > for next-generation sequencing

Introduction

in hands-on time. In addition to and measured in replicates of 2, 6, and the latest Qubit 1X dsDNA HS and BR assays.

Product name

Instrument concentration Size Cat. No.

System check

Qubit 4 System Verification Assay Kit Qubit Flex System Verification Assay Kit

Absorbance2.0

2,000 12.5 125 1,500 2,000 5,000 20,000

Compatibility

Detergents, reducing agents Reducing agents

Light sources

Blue LED (peak ~470 nm), Blue LED (peak ~460–480 nm),

buffer in a 1

200 ratio. Qubit 1X dsDNA assay kits eliminate this step by providing a ready-to-use

Initial sample

Quantitation

User interface

5.7 in. color touchscreen 8 in. color touchscreen

APPLICATION NOTE

Qubit Flex Fluorometer TECHNICAL NOTE Qubit 1X dsDNA assays

Application note

Accurate and Application note: Qubit RNA Technical note: Qubit 1X dsDNA

Detection ranges

high-sensitivity and broad-range assays

Emission filters

Green (510–580 nm), Green (513–563 nm),

Fluorometer mode

Yes No

has a new member

the Qubit™Flex fluorometer was recorded. The sample dye stockDMSO Workingsolution

Qubit Assay Tubes

500 tubes Q32856

Sample throughput

1 sample in 3 seconds 1 to 8 samples in 3 seconds

Type d’appareil

Fluorimètre de paillasse

working solution.

and Qubit Flex fluorometers. They are expected to last

Excitation filters

Blue LED (430–495 nm), Blue LED (456–484 nm),

Quantitation range

100 μg/mL to 20 mg/mL 12.5 μg/mL to 5 mg/mL

Red (665–720 nm)

Far-red (671–693 nm)

Standards dilution

Sample dilution

based instruments.

firmware for Qubit 4 and Qubit Flex fluorometers will

DNA reference guide

Sample RNA reference guide: Sample Technical note: Understanding

Entrée électrique

12 VDC, 2.5 A

Onboard calculators

Reagent calculator Reagent calculator

Qubit 4 Fluorometer

Qubit Flex Fluorometer

Sample data storage

1,000 samples 10,000 samples

modern laboratories

single-sample readers is significantly DNA was diluted to 4 concentrations performance of the standard Invitrogen Qubit dsDNA

of three components

a blank reagent solution, a green

Instrument footprint

13.6 x 25 x 5.5 cm 1.86 x 28.2 x 10.3 cm

Qubit 1X dsDNA assays

simplified workflow and

Qubit ssDNA Assay Kit

0.05–0.2 1–200 100 Q10212

improves the speed of

target molecule. (Cat. No. Q33231) was tested on the little as 1 μL of sample. Qubit dsDNA assay kits are intended to be used within

site d’installation

Utilisation en intérieur uniquement

Qubit Flex Fluorometer

1 instrument Q33327

Qubit RNA BR Assay Kit

1 ng/μL to 1 μg/μL 20–1,000 ng

Qubit RNA HS Assay Kit

250 pg/μL and 100 ng/μL 5–100 ng

Qubit RNA IQ Standards

1 set Q33235

Qubit RNA XR Assay Kit

10 ng/μL and 10,000 ng/μL 200–10,000 ng

Red LED (600–645 nm)

Red LED (612–644 nm)

Red LED (peak ~635 nm)

Red LED (peak ~620–640 nm)

Sample preparation and

Sample preparation and

purification solutions

purification solutions

Programable open format

Yes—MyQubit No

Qubit Protein Assay Kit

Qubit Flex, Qubit 4 12.5 μg/mL to 5 mg/mL

500 Qubit dsDNA HS assay

10 Qubit dsDNA BR assay Use high-sensitivity

8 samples simultaneously

have been formulated to cover a of samples, the Invitrogen™ Qubit instruments and assays enable rapid and reliable working solution can be used reliably for 6 months after

Informs where the sample

Provides quantification data for samples that are within the core and the extended range of the

Qubit Flex Fluorometer >

assessment >

Qubit dsDNA BR Assay Kit

0.2–2,000 4–2,000

Qubit dsDNA HS Assay Kit

0.005–120 0.1–120

Qubit microRNA Assay Kit

50 ng/mL to 100 μg/mL 1–1,000 ng

increases quantification

assay reagents are available for Speed of quantification polymerase chain reaction (PCR). Often, fluorometry is buffer (Figure 1). The 1X formulations enable users to simply

Interface d’utilisation

Ecran tactile couleur de 5,7 pouces

Humidité d’exploitation

20-80% (sans condensation)

Qubit Flex NGS Starter Kit

1 kit Q45893

Qubit Protein BR Assay Kit

Qubit 4 100 μg/mL to 20 mg/mL

Instruments and accessories

Unit size Cat. No.

Puissance de fonctionnement

100-240 VAC, 1.0 A/1.2 A

Qubit 1X dsDNA BR Assay Kit

0.2–4,000 4–4,000

Qubit 1X dsDNA HS Assay Kit

0.005–120 0.1–120

Qubit Flex Assay Reservoirs

100 reservoirs Q33253

Actual concentration (ng/mL)

Actual concentration (μg/mL)

Qubit Flex Assay Tube Strips

125 tube strips Q33252

concentration resides within

standard curve. Sample concentrations that are out of range are not given a measurement.

emits fluorescence only when

or protein from 1–8 samples simultaneously. (BR) Assay Kits include a fluorogenic dye, buffer, and dsDNA standards. Prior to each assay, a

preparation and purification

preparation and purification library quantification assays

rapid quantitation, and high

4.0 4,000 BCA

Qubit™assay reagents, which

Figure 1. The Qubit Flex Fluorometer

Flex Fluorometer is a benchtop

Standards dilution Sample dilution

Qubit 4 Fluorometer (w/ Wi-Fi)

1 instrument Q33238

Température de fonctionnement

10-40° C

Qubit™family of fluorescence-

Fluorometer, Qubit Flex Fluorometer,

quantification of DNA, RNA, and

Standards(10 μL) (1–20 μL)Samples

such as reducing reagents (DTT,

1.0 1,000 Sample concentration (μg/mL)

Qubit™4 Fluorometer, the Qubit

solution volume was 190 μL. B Qubit 1X dsDNA assay

based quantification instruments

and another supplier’s single-channel Buffer

samples simultaneously using the

fluorometric RNA quality improved performance >

β-mercaptoethanol), salts, free

0.0 0

Qubit 4 Fluorometer and the Qubit

3 hours of preparation.

or protein in less than 3 seconds

Figure 1. Qubit Flex and Qubit 4 fluorometers with intuitive

quantification easy and fast. The

BCA Qubit Protein BR Assay

use highly specific Invitrogen™

solution

wide range of samples compared to

Figure 10. Quantification ranges of protein assays.

Qubit 4 NGS Starter Kit (w/ Wi-Fi)

1 kit Q33240

and the new Qubit Flex Fluorometer

1X working

Fluorometer. Like the Invitrogen™

volume was 10 μL and the working

Qubit Flex Quantitation Starter Kit

1 kit Q45894

device designed for highly accurate

Standard 1Standard 2

on your sample volume and estimated

Figure 3. Reagent and assay range calculators. Easily make working solutions for all assays

Dimensions de l’appareil(L x L x H)

13.6 x 25 x 5.5 cm5.4 x 10 x 2.2 in.

Qubit 4 RNA IQ Starter Kit (w/ Wi-Fi)

1 kit Q33241

Qubit 4 System Verification Assay Kit

50 assays Q33237

protein. Both the Qubit 4 Fluorometer

Ready-to-use

Qubit 1X dsDNA HS Assay Lambda Standard

- - - Q33233

assays provide low protein variability,

5.0 5,000

Qubit 1X dsDNA BR Assay Lambda Standards

- - - Q33263

Qubit Flex System Verification Assay Kit

50 assays Q33254

precise quantification of up to 8 IQ Assay

a fast and easy assays: simplified workflow and

Qubit 4 Quantitation Starter Kit (w/ Wi-Fi)

1 kit Q33239

DNA and RNA concentrations. Absorbance-based

that contain up to 5% detergents. The Qubit Protein Assay

detection ranges, and one microRNA assay kit

miRNA

Q. Do I have to use new standards every time?

fluorometer is functioning properly?

Qubit assays are fluorescence-based. They are

options not available on my Qubit 4 or Qubit

processing quantificationsamples by up to 50%

The Qubit Flex Fluorometer increases Flex Fluorometer. The Invitrogen™ The Invitrogen™Qubit™4 and Flex Fluorometers both use™ ™

Q. I already have a Thermo Scientific NanoDrop

Q. Does the Qubit Protein Assay or Qubit Protein BR

Qubit 4 Protein BR Assay Starter Kit (w/ Wi-Fi)

1 kit A51292

a more sensitive dynamic range than absorbance-

A. To adhere to cybersecurity legal standards, the latest

simultaneously using the Qubit Flex Fluorometer

Introduction To further simplify the nucleic acid quantification

Concentration of DNA byQubit Fluorometer (ng/mL)0

Concentration of DNA byQubit Fluorometer (μg/mL)0

instrument; why should I use a Qubit fluorometer?

Assay work well in the presence of detergents?

calibration or to use the values from the previous

Kits offer a fast, easy-to-use, reagent-based method

A. For each assay, you have the choice to run a new

A. Qubit 4 and Qubit Flex System Verification Assay

When used with Qubit assays, Qubit fluorometers can

(such as instrument settings, software update, and system

A. NanoDrop instruments use UV absorbance to measure

A. The Qubit Protein BR Assay is compatible with samples

The Invitrogen Qubit 4 System Verification Assay Kit

fluorescent reagent, and a far-red fluorescent reagent.

a new calibration curve every time you prepare a new

A. There are two LED light sources in the Qubit 4

and significant temperature fluctuations within your

verification regularly.

the assays, the instrument, your pipetting accuracy,

instrument is suspected. It is not necessary to perform

Accurate and precise quantification of up to 8 samples

improved performance

NanoDrop spectrophotometers can detect the presence of

menu options will appear to be inactive.

RNA, and protein. The Invitrogen™ reproducible data.

48, and 96 samples on the Qubit 4 Standards(10 μL) (1–20 μL)Samples

accurately measure low concentrations of sample, while

verification). When not logged into a user profile, these

new calibration each time. As you become familiar with

Perform the system verification when a problem with the

and the Invitrogen Qubit Flex System Verification Assay

Paired with a hardware functionality test, the assay is

calibration. As you first use the instrument, perform a

to test the internal components of a Qubit fluorometer.

measurements have limitations in distinguishing between

is not recommended if detergent is present.

the performance of Qubit fluorometers. Each kit consists

to help ensure proper functionality.

Kit are fast, easy-to-use, reagent-based assays that test

designed to test the internal components of the instrument

Qubit fluorometers and assay kits are designed to measure

DNA dye selectivity RNA dye selectivity Protein dye selectivity

have using the calibration data stored from the last time

Q. How long does the lamp last?

Qubit fluorometers use specialized curve-fitting algorithms

RNA

connectivity expected in today’s measurements compared to

reagent solution workflow. Lambda RNA, miRNA, and protein. and purification workflows. Here we demonstrate the™ ™

designed to only quantify the target analyte. Additionally,

Flex device?

of the standards used in calibration. The detection limits of

Dyes only emit signal when bound to the target.

quantification throughput with the Qubit™dsDNA HS Assay Kit

highly specific Invitrogen Qubit assays, which contain Both standard Qubit assay kits and the latest 1X assay

calculator to determine how to dilute the samples to the same concentration.

is only available on the Qubit

less, due in part to a 50% reduction (0.1, 1, 5, and 10 ng/μL) and prepared

high sensitivity (HS) and dsDNA broad range (BR) assays

needs to be prepared by diluting the dye stock in the provided buffer in a 1

200 ratio. Qubit 1X dsDNA

contain a highly selective dye that increases throughput to quantify DNA, RNA,

Figure 1. Workflow comparison for the (A) Qubit dsDNA and (B) Qubit 1X dsDNA assays.Standard Invitrogen™Qubit™dsDNA High Sensitivity (HS) and Qubit™dsDNA Broad Range

Applications

• Analyses de recherche
• Clonage
• Séquençage
• Transfection