Solus Listeria monocytogenes ELISA

Galerie et schémas techniques

Description

Caractéristiques

• Hautement fiable
• Augmentation de l’efficacité du laboratoire
• Réduction de la demande de ressources
• Réduction des déchets
• Haute efficacité lorsqu’il est automatisé
• Temps du technicien considérablement réduit
• Méthode de test de performance de l’Institut de recherche AOAC®

Spécifications techniques

P

(800) 762-4000 or 12 12 3 3

0,2ml

6 6 6 6

Day 0

Day 1 Day 2 Day 3 Day 4

100 ml

2 Read plates Read plates

30 °C

agents e.g. ALOA incubate for a

STEP 1

STEP 2 STEP 3 STEP 4 STEP 5 STEP 6 STEP 7

SUPP011

P.A.C. supplement 10 vials

Incubate

3 3 Solus Automated ProcessSolus Automated Process

25 g food

Transfer 0.2 ml to 10 ml Take 1-2 ml heat

LISM-0096

Solus Listeria monocytogenes 96 wells

e.g. ALOA

incubate for a

in 225 ml

PAC supplemented kill and test

in 375 ml

1 1

125 g food

37 °C 24-28 hrs. +ve confirm

24-28 hrs.

Incubate -ve result or

Incubation

Incubation: Boil: 37 °C, 60 min. 37 °C, 60 min. 20-25 °C, 30 min. 450 nm

Swab/sponge

Incubate Incubate 35°C +ve confirm

10 ml/100 ml

Solus Automated ProcessSolus Automated Process2

MED039-0.5KG

Palcam broth 500 g

Product Code

Product Description Pack Size

SampleSample

8 8 8 8

30 °C 24 hrs.

4 4

100 ml25 g food

Add filter Streak onto 1 esculin Read plates Read plates

A B C D E F G H

A B C D E F G H A B C D E F G H A B C D E F G H

HIGH THROUGHPUT

EASY TO IMPLEMENT,

in 90 ml/225 ml

30 °C 20 hrs. 35 °C 24 hrs.

per swab/sponge

5

Negative Control

Positive Control Blank Well Sample wells Positive (After Substrate Addition) Positive (After Stop Solution Addition)

incubation after

1 1

940 Winter Street

1111 1111 5

Half Fraser broth

5 5

PerkinElmer, Inc.

1010 1010 1 1

+ve result confirm

Solus Automated ProcessSolus Automated Process

RESULTS IN 51 HOURS

WITH A SINGLE ASSAY

Workflow Comparison

Solus Listeria monocytogenes ELISA vs BAM Chapter 10

www.perkinelmer.com

4 4 8 8

1 chromogenic plate,

Read plates or

FOR BETTER EFFICIENCY

EASY TO USE

Waltham, MA 02451 USA

2 2 6 6

LISTERIA MONOCYTOGENES

TEST MULTIPLE MATRICES

Solus Automated Proces6

6 35°C +ve confirm

BLEB + Pyruvate Incubate

selective 1 chromogenic plate, Read plates or

Solus Automated Process8

8

the capacity for testing.

Efficient

1 2 3 4 5 36 7 8 9 1011 12

132 3 4 5 6 7 8 9 1011 12 3 1 2 3 4 5 6 7 8 9 31011 12 1 2 3 4 5 6 7 8 9 1011 12

1 2 3 4 5 6 7 8 9 10 11 12

1 2 3 4 5 106 7 8109 10 11 12 1 2 3 4 5 6 7 8

essential laboratory tasks.

detection ofListeria monocytogenes.

out on a Dynex DS2 instrument. A

LISM-0480 Solus Listeria monocytogenes 480 wells

the technician can walk away for

DS200 DS2 Automation 1

Half Fraser broth to supplemented

culture to into wells (plus controls) of conjugate 100 μl of substrate stop solution

up to three hours during each run,

Dynex DS2

single system processes two 96-well

FBH003/FBH005 Solus Half Fraser broth 2 kg,10 kg

Solus Automated Processfurther 24 hrs. @

-ve result or

and mix Palcam broth boiling tube Incubation

Incubation: Incubation: Read plate:

Presented in an immunoassay format, Solus Listeria

51 hours, including three hours to negative/presumptive

Solus1Listeria monocytogenes2 3 4 5 6 7 8 9 10 11ELISA12

1 2 3 4 5 6 7 8 9 10 11 12 12 12 1 2 3 4 5 6 7 8 9 10 11 12

monocytogenes ELISA is versatile and can be used manually or in

positive results post enrichment. Plus, when combined with

conjunction with automation. Performing to high standards, Solus

automation, you’ll benefit from an assay that’s:

Add sample to Transfer 0.2 ml culture Transfer1ml Pipette 100 μl sample

Add 100 μl Wash plate and add Add 100 μl of

Applications

• Contrôle de production agroalimentaires
• Tests environnementaux