RT-PCR QuantStudio 12 Flex

Gallery and technical drawings

Description

Key features

• Maximiser le débit avec un minimum de ressources
• Cinq blocs interchangeables
• Compatibilité d’automatisation
• Options de multiplexage et de chimie maximales
• Réduisez le temps de démarrage et le temps de travail
• Conformité 21CFR Part 11 et prise en charge LIMS

Technical specifications

®

OpenArray Block with AccuFill System 4471067

™

• Reduce start-up and hands-on time. Start a 12,000 data point through-holes via surface tension.

Power

100–240 V

Weight

69 kg (152 lbs)

Run time

•30 min expected (Fast 96-well block) •2 hr (gene expression)

reaction

reaction

analysis.

to you.

Expand the

resources

Resolution

As low as 1.5-fold change for single-plex As low as 2-fold change for single-plex

•Coupled

4 emission, 4 excitation (OpenArray blocks) DigitalSuite Software Digital PCR Analysis that enables duplex analysis, All QuantStudio 12K Flex system

AB Complete

AB Assurance AB Maintenance

Description

Specification Software name Description Blocks supported

QuantStudio

12K Flex Software

diagnostics

®

ment support

Applied Biosystems Assurance Service Plan Contact us

Dynamic range

To 9 logs To 7 logs

User friendly

A data analysis suite at your fingertips High-throughput capable

on-site repairs

on-site repairs remedial repairs ®

0.039 copies/rxn

0.062 copies/rxn 0.122 copies/rxn 1.210 copies/rxn

0.059 copies/rxn

0.116 copies/rxn 0.286 copies/rxn 3.060 copies/rxn

0.097 copies/rxn

0.165 copies/rxn 0.362 copies/rxn 3.508 copies/rxn

in a single run.

accurate and sensitive data collection (Figure 4). Additionally,

Excitation source

Enhanced OptiFlex system, White LED 21 CFR Part 11 Software Module Quality control system All blocks

Remote monitoring

Available to monitor up to 15 networked instruments simultaneously p-value, correlation plots, box plots, heat maps,

Applied Biosystems

service & support Ordering information

Instrument control

Instrument touch screen, networked computer, or attached computer

Block change design

Block change from front in less than 1 min; no tools or service call required QuantStudio 12K Flex system

Guaranteed next day

Guaranteed 2-day: Target 2-day: QuantStudio 12K Flex Twister II Automation Robot 4471066

Max block ramp rate

3.0°C/sec (384-well) 3.0°C/sec

Planned maintenance

+ + +

21 CFR p11 enablement

Optional software module one-click launch, and amplification curves & blocks

Dimensions (W x D x H)

50.5 cm x 67.2 cm x 73.8 cm

everything in between.

right for your project—today and in the future.

samples, and subarray.

for absolute quantitation with greater sensitivity and precision of rare

ExressionSuite Software

Gene expression analysis supporting up to All QuantStudio 12K Flex system

Hardware specifications

Software specifications

fluorescence detection.

™ ™ ®

Demonstrated sensitivity

To 1 copy To 1 copy

Parts, labor, and travel

Included Included Discount with Plus plan

TaqMan Array Card blocks

includes single-plate views blocks

Well-to-well variability

+/– 0.25°C +/– 0.75°C

96-Well Block Upgrade Kit

4453543

384-Well Block Upgrade Kit

4453545

SampleTracker v1.0 Software

•Facilitate sample tracking from 96-well to QuantStudio 12K Flex

Detection channels Decoupled

6 emission, 6 excitation Coupled: 4 emission, 4 excitation

Award-winning remote services

QuantStudio 12K Flex Real-Time PCR System with 384-Well Block 4471134

Block configurations and volume

•96-well (10–100 μL reactions), ™

Detection channels •Decoupled

6 emission, 6 excitation (96-well/Fast, 384-well, TaqMan blocks) Downstream applications accessible from QuantStudio 12K Flex software home screen

for QuantStudio 12K Flex system

compliance features

96-well, 96-well Fast, 384-well,

® TaqMan Genotyper Software Improved version of existing software product that All QuantStudio 12K Flex system

Instrument qualification services

+ – –

•384-well (5–20 μL reactions)

384-well plates OpenArray® block

TaqMan Array Card Block Upgrade Kit

4453546

Data export format User configurable

*.xls, *xlsx, *.txt and volcano plots.

methylation analysis in genomic DNA.

performing disease studies, these fixed-content gene

answers for publishing groundbreaking

384-well TaqMan® Array Cards

•35 min (384-well block, using Fast

•4 hr (genotyping)

•OpenArray Plates (33 nL reactions)

HRM Software Module for High resolution melt analysis 96-well, 384-well blocks

Instrument operating software upgrades

+ – –

The QuantStudio 12K Flex Real-Time PCR

• Maximum speed and throughput. The OpenArray format can

QuantStudio 12K Flex OpenArray AccuFill

coated with hydrophilic and hydrophobic compounds to retain reagents in

gene or splice variant in any organism.

™ ® • Pharmacogenomics panels. For researchers involved in

one-touch runs for the OpenArray block.

e-signature records for data traceability.

•Fast 96-well (15–30 μL reactions)

™

Applied Biosystems Complete Service Plan

Contact us

•TaqMan Array Cards (~1 μL reactions)

•Illustrates mapping to OpenArray® plates

Figure 6. Easy analysis of OpenArray runs

toggle between targets, Figure 7. Analyze your digital PCR results using DigitalSuite Software

Priority phone and email access to appli-

®

QuantStudio 12K Flex Real-Time PCR System

Download from www.appliedbiosystems.com

equipped with the OpenArray block and the

ultimate confidence in your results. 3,072 through-holes for individual 33 nL reactions (Figure 3). Plates are

low- to high-throughput sample processing

• Provides a seamless switch from qPCR to digital PCR. Increase the maximum resolution of 12,000 data points. The Enhanced

many types of experiments and users, from

the white light LED provides a broad spectrum of light for

streamlined OpenArray workflow helps save

• Simple workflow. Load your samples onto the plate (Figure 1) using the with the QuantStudio™ 12K Flex system. reaction mixtures via surface tension

Instrument systems (with desktop computer)

Cat. No.

20 minutes of hands-on time per run, and no

• Integrated analysis and quality systems. Comprehensive software

produce up to 110,000 data points or more in

™ ™

with the capabilities you’ll need tomorrow

OpenArray® plates

100 files with enhanced performance time. The

blocks

with lower-throughput feasibility experiments

• Ample data storage. Know that your data are always secure. The

Take on the experiments you’ve always wanted

• Quick, simple block changes. Thermal blocks are easy to change for

standard Custom TaqMan Copy Number Assays when a

arrays.

QuantStudio 12K Flex Software for one-touch runs.

DigitalSuite Software v1.0 you can increase the precision

TaqMan SNP Genotyping Assays and 2,700 TaqMan DME

known as castPCR technology, for detection of mutations pharmacogenetics markers, including PharmaADME core

from over 4.5 million predesigned human and mouse

by competitive allele-specific TaqMan PCR technology, drugs. This panel contains all known high-value DME

with genome-wide coverage for human, over 180,000

quantification, small RNA quantification, and targeted panels contain a defined set of select autosomal and

Genotyping Assays, or Custom TaqMan SNP Genotyping

present at less than 0.1% in gDNA. markers.

Software v1.0 can analyze data from small or large

experiment files simultaneously with the push of a single

384-well, and TaqMan Array Card blocks, and enables

compliance module assists with security, auditing, and

It’s often the unexpected results in the lab that

• Five interchangeable blocks. Choose and load the thermal-cycling Enhanced OptiFlex® System

chemistry makes SNP genotyping studies easy. Choose

type genomic DNA (gDNA). These assays are powered analysis of the effects of genes on human responses to

• Copy number analysis. TaqMan Copy Number Assays

TaqMan Assays and tools are available for long noncoding confirmation of sample identity and gender within a

Feature-rich touch screen interface makes it easy to

Innovative software applications to analyze your data. Easily keep track of your samples and your results.

a rapid, economical method for mutation scanning and

• Gene expression pathway panels. For researchers

• Gene expression analysis. TaqMan Gene Expression

• Digital PCR technology. Ideal for sensitive detection of

Assays are the most comprehensive set of quantitative

rare targets such as in cancer mutations, viruses, and Life Technologies research and development scientists

analyze experiments. The graphical interface makes it

LIMS (laboratory integration management systems) or

and sensitivity of your qPCR experiments by switching

1/400 1/200 1/100 1/10 100

are designed to detect and measure DNA mutations with

pharmacogenomics, our fixed-content panel of selected

assays targeting exons for mouse, and Custom Plus and

miRNA quantification. Y-chromosome SNP markers common to commercial

gene expression assays available, providing more than

GMO detection. In digital PCR, reactions are split into panels to take your continue to develop new collections of TaqMan Assays to

QuantStudio 12K Flex Real-Time PCR System sets the new

your research progresses.

including your choice of FAM or VIC dye labels. Custom

digital readout of real-time PCR results. research to the next Most recently we’ve introduced the following novel products:

• Enhanced gene expression analysis. ExpressionSuite

• High-throughput experiment setup. Create multiple

are a collection of over 1.6 million predesigned assays

RNA quantification, miRNA profiling, pri-miRNA large biorepository setting, our fixed-content OpenArray

capabilities to help you get more from your experiments.

10

optimize your genotyping run times using a real-time PCR

templates with export preferences that direct the system

perform sophisticated statistical analysis to understand

import your plate setup files from other Applied Biosystems

projects, even 100+ runs. Quickly check data quality and

button and match them with your unique sample files. You can

screen guide you through the workflow to set up runs and

(API) allow integration with third-party systems such as

design, this is the one instrument that can take you from

With five interchangable thermal cycling blocks, you can analyze

easy to edit thermal cycling conditions for your 96-well,

custom automated platforms. The optional 21 CFR Part 11

for maximum throughput, flexibility, and scalability, the

consistent, high-quality results across different plating formats as

• MicroRNA and other noncoding RNA analysis. Innovative

• Barcoding panels. For high-throughput tracking and

• SNP genotyping. The precision of TaqMan probe–based

extremely high specificity against a background of wild- TaqMan DME assays is ideal for high-throughput

loading your OpenArray plates, and finally integrates with

• Straightforward transition to digital PCR. With

targeted discovery through confirmation and screening, and

from 1 to over 12,000 data points per run in the assay format that’s

1.3 million primer/probe sets for 23 species, in four sizes,

hundreds to thousands of mini-reactions to provide a use with Applied Biosystems real-time PCR technology.

Life Technologies is taking qPCR to the next level. Designed

require no tools, providing the ultimate in format flexibility. Achieve

QuantStudio 12K Flex Real-Time PCR System with 96-Well Block

4471087

generating up to 110,000 data points or more per 8 hour day,

®

• Real-time control for genotyping cluster analysis. You can

• Easy, automated data export. Set up experiment

workflow, intuitive software, and a state-of-the-art industrial

Scales to your research

• Intuitive interface. Easily identifiable icons on the touch

• LIMS compatibility. Open application program interfaces

DigitalSuite Software with the QuantStudio OpenArray block. From

instantly load the appropriate experiment files for one-touch

block you need for the experiment at hand. Additional blocks are

The QuantStudio™ 12K Flex Real-Time PCR System uses a

features help you easily map and track your samples, giving you the

and are arranged in 48 subarrays of 64 through-holes, with a total of

late Ct values to undetermined copy numbers, digital PCR provides a

runs to help ensure that the plates were loaded correctly.

time PCR to digital PCR mode using QuantStudio digital PCR kits and

OpenArray plate identities from their bar codes. You can

complex pipetting to load; simply add your sample and master mix and

0.1

precision and sensitivity of your experiments by switching from real-

OptiFlex System can also capture QuantStudio 12K Flex

• Impressive throughput without complicated robotics. With 384-well

Δ

QuantStudio 12K Flex Real-Time PCR System with TaqMan Array Card Block

4471089

generate over 12,000 data points per run. Integrated software tracking

QuantStudio™ 12K Flex OpenArray® plates are microscope slide–sized Figure 3. OpenArray®plate technology.

microarrays and the data quality of solution-phase reactions such as PCR

Through-holes retain 33 nL

Ultimate combination of speed, throughput, and data analysis capabilities

Hydrophobic Hydrophilic

available to scale your throughput capability to up to 12,000 data points

white light LED optics system enabling powerful, extremely

QuantStudio 12K Flex AccuFill Upgrade Kit (For existing AccuFill system users)

4471022

Applications

• Analyses pharmaceutiques